|
KMID : 0352720170410030326
|
|
Journal of Ginseng Research
2017 Volume.41 No. 3 p.326 ~ p.329
|
|
|
Molecular differentiation of Russian wild ginseng using mitochondrial nad7 intron 3 region
|
|
Li Gui Sheng
Cui Yan
Wang Hong Tao
Kwon Woo-Saeng
Yang Deok-Chun
|
|
|
Abstract
|
|
|
Background: Cultivated ginseng is often introduced as a substitute and adulterant of Russian wild ginseng due to its lower cost or misidentification caused by similarity in appearance with wild ginseng. The aim of this study is to develop a simple and reliable method to differentiate Russian wild ginseng from cultivated ginseng.
Methods: The mitochondrial NADH dehydrogenase subunit 7 (nad7) intron 3 regions of Russian wild ginseng and Chinese cultivated ginseng were analyzed. Based on the multiple sequence alignment result, a specific primer for Russian wild ginseng was designed by introducing additional mismatch and allele-specific polymerase chain reaction (PCR) was performed for identification of wild ginseng. Real-time allele-specific PCR with endpoint analysis was used for validation of the developed Russian wild ginseng single nucleotide polymorphism (SNP) marker.
Results: An SNP site specific to Russian wild ginseng was exploited by multiple alignments of mitochondrial nad7 intron 3 regions of different ginseng samples. With the SNP-based specific primer, Russian wild ginseng was successfully discriminated from Chinese and Korean cultivated ginseng samples by allele-specific PCR. The reliability and specificity of the SNP marker was validated by checking 20 individuals of Russian wild ginseng samples with real-time allele-specific PCR assay.
Conclusion: An effective DNA method for molecular discrimination of Russian wild ginseng from Chinese and Korean cultivated ginseng was developed. The established real-time allele-specific PCR was simple and reliable, and the present method should be a crucial complement of chemical analysis for authentication of Russian wild ginseng.
|
|
|
KEYWORD
|
|
|
allele-specific polymerase chain reaction, cultivated ginseng, nad7, single nucleotide polymorphism, wild ginseng
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|